4x protein loading buffer (LI-COR)

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LI-COR 4x protein loading buffer
4x Protein Loading Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4x+protein+loading+buffer/PROTEIN+LOADING+BUFFER/10__1091_slash_mbc__e25___11___0532-172-6-10
Average 96 stars, based on 482 article reviews

4x protein loading buffer - by Bioz Stars, 2026-09

96/100 stars

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Western Blot:

Article Title: Dopamine-driven increase in IL-1β in myeloid cells is mediated by differential dopamine receptor expression and exacerbated by HIV
Article Snippet: .. On the day of the Western blot, samples were thawed at RT, and mixed with 4X protein loading buffer (Licor Biosciences, Lincoln, NE) containing 10% 2-Mercaptoethanol (BME), then boiled at 95 °C for 10 min. .. Protein lysates were then separated by gel electrophoresis on Bolt Bis–Tris Plus gradient 4–12% precast gels in MOPS/SDS running buffer in a Mini gel tank (Life Technologies, Carlsbad CA).

Article Title: Investigating the functional components of YAP condensates
Article Snippet: Yes-associated protein (YAP) condensates are critical for cell survival under hyperosmotic stress, yet how these condensates execute their specific functions remains incompletely understood.. Here, we employed proximity-based proteomics to identify YAP-interacting proteins in both the diffuse and condensate-forming states.. Upon YAP condensate formation, the composition of YAPinteracting proteins changed markedly.

Article Title: Dopamine-driven increase in IL-1β in myeloid cells is mediated by differential dopamine receptor expression and exacerbated by HIV.
Article Snippet: .. On the day of the Western blot, samples were thawed at RT, and mixed with 4X protein loading buffer (Licor Biosciences, Lincoln, NE) containing 10% 2-Mercaptoethanol (BME), then boiled at 95 °C for 10 min. .. Protein lysates were then separated by gel electrophoresis on Bolt Bis–Tris Plus gradient 4–12% precast gels in MOPS/SDS running buffer in a Mini gel tank (Life Technologies, Carlsbad CA).

Incubation:

Article Title: Somatic function of the Argonaute protein Aubergine is essential for neuromuscular development and function in Drosophila
Article Snippet: Data was processed in the QIAcuity Software Suite (QIAGEN), where absolute values (copies/μL) were obtained and normalized expression derived. .. The GFP beads-immune complexes from RIP or IP were incubated directly with 4X protein loading buffer (Li-Cor) supplemented with 2-Mercaptoethanol (Sigma) and boiled at 95°C for 10 min. Proteins were separated in Mini-Protean TGX stain-free 4%–20% precast gels (Bio-Rad) under reducing and denaturing conditions. .. Proteins were transferred to an Immuno-Blot LF PVDF membrane (Bio-Rad) on a semi-dry Trans-Blot Turbo transfer system (Bio-Rad), blocked in Intercept Blocking Buffer (Li-Cor) and incubated with primary antibodies diluted in Intercept Antibody Diluent (Li-Cor) overnight at 4°C.

Staining:

Expressing:

Article Title: Cell-Free Expression of Soluble Leafhopper Proteins from Brochosomes.
Article Snippet: Brochosomes are proteinaceous nanostructures produced by leafhopper insects with superhydrophobic and antireflective properties.. Unfortunately, the production and study of brochosomebased materials has been limited by poor understanding of their major constituent subunit proteins, known as brochosomins, as well as their sensitivity to redox conditions due to essential disulfide bonds.. Here, we used cell-free gene expression (CFE) to achieve recombinant production and analysis of brochosomin proteins.

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